In a first, the United States and the NATO have featured among the threats faced by Russia in an updated security document signed by President Vladimir Putin on 31 December, said reports.
The document, titled "About the Strategy of National Security of Russian Federation", which will be used to plan the nation's defence strategy, replaces the previous version from 2009.
The earlier version, which was endorsed by then-president Dmitry Medvedev, did not mention the United States or the NATO as a threat, reported Reuters.
The new appraisal says Russia's role in solving "independent domestic and foreign" issues has caused a counter-reaction from the US and its allies, leading to "political, economical, military and informational pressure" on Russia.
"The strengthening of Russia happens against the background of new threats to the national security, which has complex and interrelated nature," the Reuters report cited an excerpt from the paper.
The document also accuses the NATO of supporting a coup d'etat in Ukraine, thereby dividing the Ukrainian society and leading to a military conflict.
The document adds that the United States has set up military-biological laboratories in Russia's neighbouring countries, a move that violates "norms of international law".